Mass Spectrometry Proteomics Course | IIT Bombay | Prof. Sanjeeva Srivastava
Course Details
| Exam Registration | 208 |
|---|---|
| Course Status | Ongoing |
| Course Type | Core |
| Language | English |
| Duration | 4 weeks |
| Categories | Biological Sciences & Bioengineering |
| Credit Points | 1 |
| Level | Undergraduate/Postgraduate |
| Start Date | 16 Feb 2026 |
| End Date | 13 Mar 2026 |
| Enrollment Ends | 16 Feb 2026 |
| Exam Registration Ends | 27 Feb 2026 |
| Exam Date | 25 Apr 2026 IST |
| NCrF Level | 4.5 — 8.0 |
Master the Tools Decoding the Proteome: A Course in Mass Spectrometry Based Proteomics
In the quest to understand the complex machinery of life, proteins stand as the fundamental functional units. While genomics provides the blueprint, proteomics reveals the dynamic, living picture of an organism. At the forefront of this revelation is mass spectrometry (MS), a powerful analytical technique that has revolutionized our ability to identify, characterize, and quantify proteins on a massive scale. If you're an undergraduate or postgraduate student in Biological Sciences or Bioengineering looking to build a strong foundation in this cutting-edge field, a specialized course led by an expert can be your gateway.
About the Course Instructor: Prof. Sanjeeva Srivastava
This comprehensive course is designed and taught by Prof. Sanjeeva Srivastava, the Group Leader of the Proteomics Laboratory at the prestigious Indian Institute of Technology Bombay (IITB). With a Ph.D. from the University of Alberta and post-doctoral training at Harvard Medical School, Prof. Srivastava brings a wealth of experience in proteomics, stress physiology, and the application of data science in global health.
Recognized for his excellence in teaching with an award from IIT Bombay, Prof. Srivastava is also a pioneer in proteomics education. He has been instrumental in developing e-Learning curriculums and Virtual Labs at IIT Bombay, initiatives that have gained international recognition and are included in the Human Proteome Organization (HUPO) International Proteomics Tutorial Programme. His vision is to link proteomics with big data for disruptive innovation, emphasizing trans-generational capacity building for the benefit of global health.
Course Overview: What You Will Learn
This 4-week course is structured to take you from the fundamental concepts to the advanced applications of mass spectrometry in proteomics. It is tailored for students with a basic background in biology and biochemistry (B.Sc./M.Sc. level).
The course begins with an introduction to proteomics and the critical steps of sample preparation, including protein extraction and digestion. You will then dive deep into the heart of the technology:
- The Fundamentals of Mass Spectrometry: Understand how MS works by producing and separating charged molecules based on their mass-to-charge (m/z) ratio.
- Key Ionization Techniques: Learn about MALDI (Matrix-Assisted Laser Desorption/Ionization) and ESI (Electrospray Ionization), the workhorses of modern proteomics.
- Chromatography & Mass Analyzers: Explore how liquid chromatography (LC) is coupled with MS and the function of different mass analyzers.
- Quantitative Proteomics: Master the principles and workflows of major quantitative techniques like SILAC (in vivo labeling), iTRAQ, and TMT (in vitro labeling).
- Data Analysis & Systems Biology: Learn how to interpret MS data and integrate proteomic findings into a broader systems biology context to understand complex biological networks.
Detailed Course Layout
The course is meticulously planned over four weeks, combining theoretical lectures with practical lab sessions to provide a holistic learning experience.
| Week | Lecture Topics | Lab Sessions (Practical Demos) |
|---|---|---|
| Week 1 | Proteomics Intro, Sample Prep, MS Fundamentals | Protein pre-fractionation, Q-TOF MS demo |
| Week 2 | Chromatography, Ionization Sources, Mass Analyzers, MALDI | Tissue preservation, Targeted proteomics (Triple Quadrupole) |
| Week 3 | Quantitative Proteomics, Hybrid MS, SILAC, iTRAQ, TMT | MS Data Analysis, Parallel Reaction Monitoring |
| Week 4 | Data Analysis, Systems Biology, Applications, Challenges | Protein-protein interaction (Label-free), SWATH-MS technology |
Who Should Enroll?
This course is ideal for:
- Undergraduate (B.Sc.) and Postgraduate (M.Sc., Ph.D.) students in Biological Sciences, Biochemistry, Biotechnology, and Bioengineering.
- Researchers and professionals seeking to solidify their understanding of mass spectrometry applications in proteomics.
- Anyone aspiring to build a career in proteomics, biomarker discovery, drug development, or systems biology.
By the end of this course, you will have gained a robust understanding of the complete mass spectrometry-based proteomics workflow—from preparing a biological sample to interpreting complex data. You'll be equipped with the knowledge to understand contemporary proteomics literature and to apply these techniques in a research setting.
Embark on this learning journey with an acclaimed instructor and institution. Explore the dynamic world of proteins and master the analytical technology that is pivotal to modern life sciences and medical research.
Enroll Now →